Co-directional replication–transcription conflicts lead to replication restart
نویسندگان
چکیده
منابع مشابه
Replication Restart after Replication-Transcription Conflicts Requires RecA in Bacillus subtilis.
UNLABELLED Efficient duplication of genomes depends on reactivation of replication forks outside the origin. Replication restart can be facilitated by recombination proteins, especially if single- or double-strand breaks form in the DNA. Each type of DNA break is processed by a distinct pathway, though both depend on the RecA protein. One common obstacle that can stall forks, potentially leadin...
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We have studied the fate of blocked replication forks with the use of the Escherichia coli priA mutant, in which spontaneously arrested replication forks persist owing to the lack of the major replication restart pathway. Such blocked forks undergo a specific reaction named replication fork reversal, in which newly synthesized strands anneal to form a DNA double-strand end adjacent to a four-wa...
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T o uphold genome stability, cells are armed with sophisticated mechanisms to promote timely and accurate duplication of the genome. For instance, stalling of the replication machinery upon encountering blockage, such as a bulky lesion, template DNA with a high tendency for forming secondary structures, or DNA-bound protein(s), ignites a DNA damage response (DDR). The DDR signaling cascade ulti...
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Multi-protein DNA replication complexes called replisomes perform the essential process of copying cellular genetic information prior to cell division. Under ideal conditions, replisomes dissociate only after the entire genome has been duplicated. However, DNA replication rarely occurs without interruptions that can dislodge replisomes from DNA. Such events produce incompletely replicated chrom...
متن کاملMUS81-EME2 Promotes Replication Fork Restart
Replication forks frequently stall at regions of the genome that are difficult to replicate or contain lesions that cause replication blockage. An important mechanism for the restart of a stalled fork involves endonucleolytic cleavage that can lead to fork restoration and replication progression. Here, we show that the structure-selective endonuclease MUS81-EME2 is responsible for fork cleavage...
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ژورنال
عنوان ژورنال: Nature
سال: 2011
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature09758